A Certified Spiking Solution® of major catecholamine metabolites 5-Hydroxyindole-3-acetic acid (5-HIAA), Homovanillic acid (HVA), and (±)-Vanilmandelic acid (VMA). This multi-component mix is suitable for use in preparation of linearity standards, calibrators, or controls as well as for other critical quantitative applications such as internal proficiency testing or for OEM use in preparation of reagents, assay development, and instrument IQ/OQ. Certified concentration values for each component provides increased efficiency from less time remaking pools due to dilution errors or rerunning samples.